Salome, J.P. and Amutha, R. and Jagannathan, P. and Joshiah, J.J.M. and Sheela, B. and Yegnaraman, V. (2009) Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum. Biosensors and Bioelectronics, 24 (12). pp. 3487-3491.

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Abstract

Thiswork describes an electrochemical method for the determination of the nitrate and nitrite reductase activities of Rhizobium japonicum. The advantage of themethod lies in the use of whole cells for the analysis and we earlier developed this protocol for the assay of NO. The results obtained are comparable to the spectrophotometric Griess assay. As the method is based on electrochemical reduction, the commonly interfering biological components like ascorbic acid, uric acid, dopamine, etc., will not interfere with the analysis. This method can be extended to the fabrication of biosensors for nitrate and nitrite using the same principle.

Item Type:	Article
Uncontrolled Keywords:	Enzyme activity; Nitric oxide; Amperometry; Cyclic voltammetry; Biosensor; Rhizobium japonicum
Subjects:	Electrochemical Analysis
Divisions:	UNSPECIFIED
Depositing User:	TTBD CECRI
Date Deposited:	01 Apr 2012 11:48
Last Modified:	01 Apr 2012 11:48
URI:	http://cecri.csircentral.net/id/eprint/255

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