Jha, N.S. and Satyendra , M. and Mamidi, S. and Ashalatha, S. and Archita , M. and Jha, S.K. and Avadhesha , S. (2016) Targeting human telomeric G-quadruplex DNA with curcumin and its synthesized analogues under molecular crowding conditions. RSC Advances, 6. pp. 7474-7487. ISSN 2046-2069

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Abstract

The formation of telomeric G-quadruplexes has been shown to inhibit telomerase activity. Indeed, a number of small molecules capable of p-stacking with G-tetrads have shown the ability to inhibit telomerase activity through the stabilization of G-quadruplexes. Curcumin displays a wide spectrum of medicinal properties ranging from anti-bacterial, anti-viral, anti-protozoal, anti-fungal and anti-inflammatory to anti-cancer activity. We have investigated the interactions of curcumin and its structural analogues with the human telomeric sequence AG3(T2AG3)3 under molecular crowding conditions. Experimental studies indicated the existence of a AG3(T2AG3)3/curcumin complex with binding affinity of 0.72 � 106 M1 under molecular crowding conditions. The results from UV-visible absorption spectroscopy, a fluorescent TO displacement assay, circular dichroism and molecular docking studies, imply that curcumin and their analogues interact with G-quadruplex DNA via groove binding. While other analogs of curcumin studied here bind to G-quadruplexes in a qualitatively similar manner their affinities are relatively lower in comparison to curcumin. The Knoevenagel condensate, a methoxy-benzylidene derivative of curcumin, also exhibited significant binding to G-quadruplex DNA, although with two times decreased affinity. Our study establishes the potential of curcumin as a promising natural product for G-quadruplex specific ligands.

Item Type:	Article
Uncontrolled Keywords:	human telomeric; G-quadruplex DNA;curcumin;molecular crowding
Subjects:	Industrial Metal Finishing
Divisions:	UNSPECIFIED
Depositing User:	Dr. N Meyyappan
Date Deposited:	11 Aug 2017 07:37
Last Modified:	11 Aug 2017 07:37
URI:	http://cecri.csircentral.net/id/eprint/3187

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