Vijayalakshmi, A. and Karthikeyan, R. and Sheela, B. (2010) Nonenzymatic Reduction of Hydrogen Peroxide Produced during the Bioelectrocatalysis of Glucose Oxidase on Urchin-like Nanofibrillar Structures of Cu on Au Substrates. Journal of Physics and Chemistry, 114. pp. 22159-22164.

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This work describes the nonenzymatic reduction of hydrogen peroxide on urchin-like nanofibrillar structures of Cu formed on gold substrates modified by a self-assembled monolayer (SAM) of p-mercaptobenzoic acid. Because the detection is based on the electrochemical reduction, the method is less prone to interference from easily oxidizable biologically important compounds such as dopamine, uric acid, and ascorbic acid. This method is extended to the detection of glucose by immobilizing glucose oxidase on the nanocomposite electrode with the help of chitosan. The linear detection range for glucose is 100 nM to 1800 nM. Sensitivity of detection is 1.99 nA/nM. The Michaelis-Menten constant is very low (125 nM), indicating high binding affinity of the enzyme to the substrate.

Item Type: Article
Subjects: Electrodics and Electrocatalysis
Depositing User: ttbdar CECRI
Date Deposited: 20 Jan 2012 23:15
Last Modified: 20 Jan 2012 23:15

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