Dhandapani, P. and Muralikannan, M. and Anandkumar, B. and Maruthamuthu, S. and Manoharan, SP. (2014) Electrochemistry of calcium precipitating bacteria in orthodontic wire. Oral Science International, 11. pp. 22-29. ISSN 1348-8643

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Abstract

Calculus composed of inorganic and organic components with bacteria formed on teeth getsdeposited on orthodontic wires. The reason for calculus formation and impact of calcium precipitatingbacteria (CPB) on orthodontic wire were studied. A pilot study on electrochemical characterization ofCPB on orthodontic wires was done.Methods: CPB were isolated from orthodontic patients and identified by molecular techniques. The elec-trochemical behavior of two isolates (CPB-1 and CPB-3) on orthodontic wires was studied by employingpolarization and impedance techniques. The CPB morphology by scanning electron microscopy and chem-ical characterization of CPB and tooth pulp stone were studied by Fourier transform infrared (FTIR) andX-ray diffraction (XRD).Results: The two isolates Bacillus megaterium (CPB-1) and Paenibacillus sp. (CPB-3) identified with 16SrRNA sequencing method increased pH of B4 medium from 5.32 to 8.3. The carboxylic acid and phosphategroups identified in FTIR analysis acted as nucleation sites for calcium deposition. The biogenic crystalphases identified in teeth pulp stone by XRD were similar to bacterial isolates cultured in the laboratory.The electrochemical studies with two CPB species revealed that biogenic calcium phosphate species actas cathodic inhibitors on orthodontic wire.Conclusion: The present study concluded that teeth pulp stone formation is due to CPB and high pHdetermines the mineralization process. Diffusion process and dispersive capacitive behavior indicate thatthe chloride ions may penetrate through calcium deposits and initiate pitting corrosion on orthodonticwire which may enhance the leaching of toxic elements in saliva.

Item Type:	Article
Uncontrolled Keywords:	Calcium precipitating bacteria; Microbial corrosion; Orthodontic wire; Polarization; Impedance techniques
Subjects:	Biocorrosion Microbiolcorrosion
Divisions:	UNSPECIFIED
Depositing User:	Dr. N Meyyappan
Date Deposited:	20 Sep 2017 11:10
Last Modified:	20 Sep 2017 11:10
URI:	http://cecri.csircentral.net/id/eprint/3199

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