Subrahmanyam, S. and Kodandapani, N. and Shanmugam, K. and Moovarkumuthalvan, K. and Jeyakumar, D. and Subramanian, T.V. (2001) Development of a sensor for acetic acid based on fusarium solani. Electroanalysis, 13 (15). pp. 1275-1278. ISSN 1040-0397

PDF Restricted to Registered users only Download (76Kb) | Request a copy

Abstract

Electrochemical biosensors have become very important tools in analytical chemistry because of their advantages like accuracy, high sensitivity and easy handling. This article describes electrochemical detection of acetic acid based on the fungus Fusarium solani. The biosensor employed for the study of assimilation of substrates was fabricated by coupling the immobilized membrane with the DO probe using dialysis tubing. A microbial dispersion containing 0.2 g of wet weight of the organisms per mL was used for immobilization upon cellulose nitrate membrane. The membrane retaining the fungus was placed on the Teflon membrane of the oxygen electrode so that the fungus was trapped between the two membranes. The linear range was found to be between 2 and 70 ppm (v/v) of acetic acid. A polypropylene net increases the lifetime of the sensor due to its retention of humidity in the net. By dipping the electrochemical probe onto buffer (pH 7.2), the sensor was found to have an extended lifetime of 120 days with about 800 determinations

Item Type:	Article
Uncontrolled Keywords:	Acetic acid; Biosensors; Fusarium solani; Isolation; Assimilation
Subjects:	Electrodics and Electrocatalysis
Divisions:	UNSPECIFIED
Depositing User:	ttbdu cecri
Date Deposited:	06 Apr 2012 15:51
Last Modified:	06 Apr 2012 15:51
URI:	http://cecri.csircentral.net/id/eprint/1092

Actions (login required)

View Item