Subrahmanyam, S. and Shanmugam, K. and Muralimadhav, V. and Murugesan, M. and Subramanian, T.V. and Jeyakumar, D. (2001) Voltammetric studies of aspergillus niger. Assessment of their growth using electroactive extracellular production of metabolite. Electroanalysis, 13 (11). pp. 1051-1054. ISSN 1040-0397

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Abstract

Large number of biochemical activities and electron behavior have a very close relationship with life forms and living cells [1], which makes the study of physiological and biochemical characteristics an important area in analytical chemistry. In recent years, researchers have developed rapid methods of assessment of cell proliferation [2–3], including reports on methods involving ATP bioluminescence [4], antibody-direct epifluorescent filter technique [5], enzyme immunoassays [6, 7], polymerase chain reaction [8], resonant mirror biosensor [9], quartz crystal microbalance biosensor [10], dielectric measurement [11], electron transfer in protein [12], and the electric impedance sensing systems [13]. The applications of direct voltammetry of cell cultures in the detection of viable microbes in biological samples have been reported [14–16]. Recent research has demonstrated that the live microbes, Tetrahymena shanghaiensis [17] and the Saccharomyces cerevisiae [18], could be monitored using cyclic voltammetry (CV). An attempt has been made here to extend this for fungal strains. The conventional method of the studies on the growth phases of fungi is cumbersome and time consuming because the time period required for the growth of the fungi is in terms of days, while for bacterial strains, all the phases of growth are completed in a few hours. This manuscript discusses the voltammeric behavior of the fungus, Aspergillus niger that was isolated from polyurethane waste scraps.

Item Type:	Article
Uncontrolled Keywords:	Aspergillus niger; Cyclic voltammetry; Growth
Subjects:	Electrodics and Electrocatalysis
Divisions:	UNSPECIFIED
Depositing User:	ttbdu cecri
Date Deposited:	06 May 2012 06:37
Last Modified:	06 May 2012 06:37
URI:	http://cecri.csircentral.net/id/eprint/1089

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